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Chinese Journal of High Pressure Physics  > 2005  >  19(4): 305-311 .
XU Shi-Ping, GUO Li-Xiu, WENG Ke-Nan, DUAN Jun, Lü Guang-Cai. Pressure Induced Rice Mutation and ISSR Analysis of the Mutants[J]. Chinese Journal of High Pressure Physics, 2005, 19(4): 305-311 . doi: 10.11858/gywlxb.2005.04.004
Citation: XU Shi-Ping, GUO Li-Xiu, WENG Ke-Nan, DUAN Jun, Lü Guang-Cai. Pressure Induced Rice Mutation and ISSR Analysis of the Mutants[J]. Chinese Journal of High Pressure Physics, 2005, 19(4): 305-311 . doi: 10.11858/gywlxb.2005.04.004
shu

Pressure Induced Rice Mutation and ISSR Analysis of the Mutants

doi: 10.11858/gywlxb.2005.04.004
  • 1.

    Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China;

  • 2.

    South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China

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  • Corresponding author: XU Shi-Ping
  • Received Date: 15 Oct 2004
  • Rev Recd Date: 07 Dec 2004
  • Publish Date: 05 Dec 2005
    Abstract
  • After up to 10 generations of purification by strict self-fertilizing, seeds of two rice varieties (Oryza sativa L.), Yuexiangzhan and Bijing-38 were treated with high hydrostatic pressure during last 5 years, and 5 contemporaneous mutants and 4 descendant natural mutants were obtained with high reproducibility for the contemporaneous mutants. To verify if the mutation was take place at DNA level, Inter Simple Sequence Repeat (ISSR) analysis of the original rice varieties and mutants was conducted. The analysis revealed that the genetic substance (DNA) had been altered. Evidently, the mutation was induced by high hydrostatic pressure treatment. Generally, the mutation took place contemporaneously and the new characters are of high genetic stability; the further the genetic distance between the mutant and the original is, the higher the genetic stability of the mutant is.

     

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