Effects of High Hydrostatic Pressure (HHP) with Bamboo Vinegar on the Quality and Protein Characteristics of Perch (Lateolabrax japonicus) Fillets during Refrigerated Storage
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摘要: 为分析超高压结合竹醋液处理对冷藏鲈鱼片品质变化与蛋白特性的影响,将鲈鱼片分别用0%、1%、2%和3%竹醋液浸渍后,进行250 MPa、9 min超高压处理,随后置于4 ℃贮藏。分别在第0、4、8、12、14、16、18 d对各组样品进行理化指标(色差、pH值、电导率、TVB-N值)、感官及蛋白质特性(肌动球蛋白含量、总巯基含量、TCA可溶性寡肽溶出量)分析,综合评价不同处理方式对冷藏鲈鱼片品质与蛋白特性的影响。分析结果表明:超高压结合3%竹醋液处理后,鲈鱼片的TVB-N值在贮藏末期(18 d)达(31.88±1.33) mgN/100 g,明显低于超高压冷藏对照组样品(44.77±1.89) mgN/100 g;此外,该处理方式还能延缓样品pH值变化和电导率的升高;蛋白特性指标显示该处理同时显著减缓了样品中蛋白质的降解变性,并维持其良好特性。因此,超高压结合竹醋液处理能够明显改善鲈鱼片的综合品质并维持其蛋白特性,其中超高压结合3%竹醋液处理的效果最优,能使冷藏鲈鱼片的货架期至少延长4 d。Abstract: In this study we aimed to study the effects of high hydrostatic pressure (HHP) with bamboo vinegar (BV) on enhancing the quality and improving the protein properties of perch (Lateolabrax japonicus) fillets over refrigerated storage. We treated fillet samples stored under refrigerated conditions at 4 °C using HHP (250 MPa, 9 min) along with 0, 1%, 2%, 3% BV respectively. We calibrated the physicochemical qualities (color difference, pH value, electrical conductivity and total volatile basic nitrogen (TVB-N) value) and examined the changes that occurred in terms of the sensory impressions and protein properties (actin globulin content, total sulfhydryl content, TCA soluble oligopeptide elution) of the samples on the 1st, 4th, 8th, 12th, 14th, 16th, and 18th day respectively during the storage time. The results showed that the samples exhibited obvious quality improvement: the TVB-N value of the samples treated by HHP with 3% BV was (31.88±1.33) mgN/100 g on 18th day, significantly lower than that of CK group ((44.77±1.89) mgN/100 g). In addition, the treatment also reduced the increase of the pH value and electrical conductivity, thereby significantly slowing down the protein degradation and maintaining its good characteristics. Finally, the shelf life of the perch fillets thus treated in cold storage was prolonged by four days.
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表 1 鲈鱼QIM感官评价表
Table 1. QIM scheme for sensory evaluation standards of Lateolabrax japonicus
QI value Sensory description Color Odour Texture 0 Normal color Neutral Rubbery 1 Color turned white Slight Fishy Malleable 2 Pale color Heavily fishy Soft 表 2 超高压结合竹醋液处理对冷藏鲈鱼片色差变化的影响
Table 2. Effect of HHP with BV on the change of L* value of Lateolabrax japonicas fillets during refrigerated storage
Storage time/d L* value HHP HHP+1%BV HHP+2%BV HHP+3%BV 0 54.72±1.375bcC 62.31±0.382abA 59.75±0.144aB 59.96±0.168bcB 4 61.59±4.758abA 57.95±1.846bA 56.81±1.621aA 60.10±1.387bcA 8 61.28±6.349abA 60.27±1.353abA 59.56±2.768aA 56.55±2.180cA 12 62.64±0.526aAB 58.47±0.210bB 60.99±3.173aB 66.14±3.486aA 14 53.57±1.326cC 64.70±1.812aA 58.97±3.627aB 65.74±1.006aA 16 60.14±5.480abcA 62.53±2.249abA 57.99±1.134aA 60.67±4.771abcA 18 61.65±1.522abA 60.43±6.603abA 57.88±1.680aA 59.71±4.329abA -
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