Effect of High Pressure Processing with Different Holding Time on the Quality of Pomfret (Pampus argenteus) Fillets
doi: 10.11858/gywlxb.20180549
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摘要: 为研究保压时间对超高压鲳鱼片冷藏期间品质变化的影响,以未做任何处理样品作为对照组(CK),研究在200 MPa的超高压条件下,通过10 min(HPP10)、20 min(HPP20)与30 min(HPP30)的保压时间处理后样品的品质变化,每隔2 d分别对各组样品的微生物指标(Total Viable Count,TVC)、理化指标(pH值、总挥发性盐基氮(Total Volatile Base Nitrogen,TVB-N)、色差、持水力(Water Holding Capacity,WHC)与质构分析(Texture Profile Analysis,TPA)与感官分值进行测定。结果表明,样品经过超高压处理后,其在贮藏期间的微生物生长、蛋白质降解与汁液流失得到明显抑制,其质构特性得以保持。指标间相关性分析结果显示,△E、pH值、TVB-N和TVC等相关性好。经200 MPa、30 min超高压处理能使冷藏鲳鱼片的货架期由4 d延至8~12 d。Abstract: The aim of this work was to evaluate the effect of high pressure processing (HPP) with different holding time on the quality of pomfret (Pampus argenteus) during cold storage. Different processing times (10, 20 and 30 min) at 200 MPa were applied and HPP treated pomfret were compared with untreated samples. Microbiological parameter (total viable count (TVC)), physicochemical (pH and total volatile base nitrogen (TVB-N), color difference, water holding capacity (WHC), texture profile analysis (TPA)) and sensory evaluation were performed respectively after the application of high pressure treatment on pomfret at the interval of 2 d (2 days).The significant difference (P < 0.05) regarding the results have been observed between HPP and CK group after 2 d storage on the values of TVC, pH, TVB-N, △E(Total color difference), WHC, TPA and sensory score. The controlled groups were the first to present signs of degradation reaching rejection[JP]threshold values for all evaluated parameters. The shelf life of CK group was only 4-5 d. HPP treatment has shown to be effective in inhibiting microorganism growth, protein degradation and the drip loss in samples and the texture characteristic of samples can be better maintained. The results of correlation analysis of different groups suggested that the correlation between △E, pH, TVB-N and TVC were significant. The shelf-life of treated pomfret samples can be extended from 4 d to 8-12 d and the optimal condition of fresh keeping effects was 200 MPa and 30 min.
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Key words:
- high pressure processing (HPP) /
- pampus argenteus /
- refrigerated storage /
- quality change
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Table 1. Sensory evaluation of pomfret (Pampus argenteus) fillets
Best(5) Extremely bright Fresh flavor Extremely firm Rich in elasticity Better(4) Very bright Fresh seaweed flavor Very firm Very elasticity Normal(3) Moderately bright Moderate seaweed flavor Moderately firm Moderate elasticity Worse(2) Slightly dull No flavor Slightly soft Slightly elasticity Worst(1) Very dull Spoiled flavor Very soft Inelastic Table 2. Variations of pH value, TVB-N, Δ E and WHC in pomfret fillets during refrigerated storage
Storage time/d Storage time/d TVB-N/(mgN/100g) ΔE WWHC/% CK HPP10 HPP20 HPP30 CK HPP10 HPP20 HPP30 CK HPP10 HPP20 HPP30 CK HPP10 HPP20 HPP30 0 6.92±0.02bB 6.91±0.01bC 6.90±0.02aA 6.92±0.01aA 19.24±1.98aA 18.16±0.51aB 14.49±2.11aC 13.93±0.89abC 51.23±0.68aA 47.22±6.30aB 41.88±0.42bB 38.80±1.55bC 9.23±1.01bC 11.05±1.48bB 12.47±1.33bB 15.04±1.29bA 2 6.86±0.00bcC 6.88±0.05aA 6.87±0.06aB 6.90±0.01aB 23.49±1.80acB 19.72±1.87acC 15.70±0.74acA 14.24±0.96abA 52.05±2.29aA 47.53±0.81aB 44.79±1.21bB 40.07±2.25bC 9.04±1.36bC 10.29±1.95bB 11.16±1.33bB 14.36±1.27bA 4 6.87±0.00bcB 6.86±0.02aC 6.82±0.01bA 6.88±0.06bA 30.48±4.07bA 21.20±1.27bC 19.69±0.10bB 17.31±0.64cB 53.56±4.00aA 49.47±1.26aB 46.81±0.86abB 43.59±2.15abC 8.74±1.32cC 9.56±1.79aA 10.22±1.39cA 12.41±1.12cB 6 7.00±0.01aA 6.94±0.00bC 6.84±0.00bB 6.90±0.01bB 35.53±3.65cA 27.23±1.54cC 23.50±2.35cB 22.06±0.88abB 55.39±0.02abA 53.20±1.14abB 49.88±3.81aB 46.14±1.71aC 7.11±1.25bB 8.39±1.27bA 9.31±1.38bA 11.23±1.52bC 8 7.15±0.01aA 6.74±0.01cC 6.72±0.01cB 6.78±0.04cB 39.45±3.90bA 30.47±0.42bC 28.02±0.66bB 24.67±0.65cB 60.45±0.76abA 57.47±2.69abB 55.46±4.67aB 53.68±2.52aC 6.31±1.47aC 8.06±1.21cA 9.28±1.14aA 10.57±1.51aB 10 7.27±0.04cA 6.62±0.01bC 6.69±0.01bB 6.63±0.02bB 45.10±2.82aA 38.16±0.60aC 31.92±1.05aB 26.71±1.80aB 67.88±2.43bA 59.13±2.02bB 57.91±4.85cB 55.59±1.82aC 5.22±1.06cC 7.31±1.22aB 7.47±1.25cB 9.42±1.03cA 12 7.39±0.022cA 6.62±0.01cC 6.66±0.04cB 6.67±0.00cB 49.44±4.42aB 45.40±2.04aA 37.87±6.32aC 28.12±0.61aC 70.29±5.62bA 61.72±1.22bB 61.41±2.29aB 59.48±2.31aB 5.04±1.43aC 6.25±1.61cB 7.28±1.07aB 9.09±1.24aA Note:(1) Values and standard deviations from three replicates are presented.
(2) Different small letters (a, b, c) in same row are significantly different (P < 0.05).
(3) Different capital letters (A, B, C) in same column are significantly different (P < 0.05).Table 3. Correlation analyses between Δ E, pH value, TVB-N and TVC of different groups
Group Correlations WHC ΔE pHvalue TVB-N CK ΔE 0.117 pH 0.364 0.132 TVB-N -0.249 -0.555** -0.565** TVC -0.133 -0.452* 0.884** HPP10 ΔE -0.090 pH 0.083 -0.686** TVB-N 0.025 0.799** -0.663** TVC -0.097 -0.534* -0.931** 0.768** HPP20 ΔE -0.271 pH 0.014 -0.717** TVB-N 0.017 0.765** -0.928** TVC 0.054 -0.555* -0.933** 0.980** HPP30 ΔE 0.521* -0.575** pH 0.330 0.582** TVB-N -0.245 -0.494* -0.891** TVC -0.038 -0.422 -0.869** 0.839** Notes:(1)** means the correlation is significant at the 0.01 level (2-tailed).
(2)* means the correlation is significant at the 0.05 level (2-tailed). -
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